Primary structure of rabbit sperm protamine, the first protamine of its type with an aberrant N‐terminal
- 19 December 1988
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 242 (1) , 111-116
- https://doi.org/10.1016/0014-5793(88)80996-7
Abstract
Rabbit protamine was extracted from S-(pyridylethylated) sperm cell nuclei with hydrochloric acid and then isolated by reversed-phase HPLC. The primary structure was determined by amino acid sequence analysis of the total protein and of fragments obtained by digestion with endoproteinase Lys-C and thermolysin. The protamine contains 49 amino acid residues and is clearly homologous with mammalian type 1 protamines, 47% of the positions being invariant. Surprisingly, rabbit protamine possesses an N-terminal valine residue, whereas all mammalian and several non-mammalian protamine sequences of this type start with alanine, the N-terminal region being remarkably conserved during evolution.Keywords
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