Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Top Cited Papers
- 1 December 2001
- Vol. 25 (4) , 402-408
- https://doi.org/10.1006/meth.2001.1262
Abstract
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This publication has 10 references indexed in Scilit:
- An Overview of Real-Time Quantitative PCR: Applications to Quantify Cytokine Gene ExpressionMethods, 2001
- Quantitation of Viral Load Using Real-Time Amplification TechniquesMethods, 2001
- Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCRJournal of Biochemical and Biophysical Methods, 2000
- Quantitative Reverse Transcription–Polymerase Chain Reaction to Study mRNA Decay: Comparison of Endpoint and Real-Time MethodsAnalytical Biochemistry, 2000
- Development and Validation of Real-Time Quantitative Reverse Transcriptase–Polymerase Chain Reaction for Monitoring Gene Expression in Cardiac Myocytesin VitroAnalytical Biochemistry, 1999
- The Transcriptional Program in the Response of Human Fibroblasts to SerumScience, 1999
- Real time quantitative PCR.Genome Research, 1996
- Selective degradation of early-response-gene mRNAs: functional analyses of sequence features of the AU-rich elements.Molecular and Cellular Biology, 1994
- Use of the polymerase chain reaction in the quantitation of MDR-1 gene expressionBiochemistry, 1990
- Quantitative analysis of MDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction.Proceedings of the National Academy of Sciences, 1990