Ilabahen Patel
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Interests
Ilabahen Patel has a total of 15 co-authors. Here are the top co-authors by number of shared publications:
- Bryan J. Harper
Department of Environmental and Molecular Toxicology Oregon State University Corvallis OR USA
1 shared publication - Christie M. Sayes
Baylor University
1 shared publication - Douglas M. Fox
Department of Chemistry, American University, Washington, USA
1 shared publication - Glen M. DeLoid
Nanoscience and Advanced Materials Center, Environmental and Occupational Health Sciences Institute (EOHSI), Rutgers Biomedical Health Sciences, Rutgers University, Piscataway, NJ 08854, USA
1 shared publication - Jeffrey W. Gilman
Material Measurement Laboratory National Institute of Standards and Technology Gaithersburg MD 20899 USA
1 shared publication - Jeremiah W. Woodcock
Materials Science and Engineering Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, United States
1 shared publication
Latest Publications
- Open Access
- 15 April 2021
- Published by MDPI AG in Nanomaterials
- Vol. 11 (4) , 1015
Fluorescently Labeled Cellulose Nanofibers for Environmental Health and Safety Studies
An optimal methodology for locating and tracking cellulose nanofibers (CNFs) in vitro and in vivo is crucial to evaluate the environmental health and safety properties of these nanomaterials. Here, we report the use of a new boron-dipyrromethene (BODIPY) reactive fluorescent probe, meso-DichlorotriazineEthyl BODIPY (mDTEB), tailor-made for labeling CNFs used in simulated or in vivo ingestion exposure studies. Time-correlated single photon counting (TCSPC) fluorescence lifetime imaging microscopy (FLIM) was used to confirm covalent attachment and purity of mDTEB-labeled CNFs. The photoluminescence properties of mDTEB-labeled CNFs, characterized using fluorescence spectroscopy, include excellent stability over a wide pH range (pH2 to pH10) and high quantum yield, which provides detection at low (μM) concentrations. FLIM analysis also showed that lignin-like impurities present on the CNF reduce the fluorescence of the mDTEB-labeled CNF, via quenching. Therefore, the chemical composition and the methods of CNF production affect subsequent studies. An in vitro triculture, small intestinal, epithelial model was used to assess the toxicity of ingested mDTEB-labeled CNFs. Zebrafish (Danio rerio) were used to assess in vivo environmental toxicity studies. No cytotoxicity was observed for CNFs, or mDTEB-labeled CNFs, either in the triculture cells or in the zebrafish embryos.
- 15 April 2021
- Published by MDPI AG in Nanomaterials
- Vol. 11 (4) , 1015
Open AccessFluorescently Labeled Cellulose Nanofibers for Environmental Health and Safety Studies
An optimal methodology for locating and tracking cellulose nanofibers (CNFs) in vitro and in vivo is crucial to evaluate the environmental health and safety properties of these nanomaterials. Here, we report the use of a new boron-dipyrromethene (BODIPY) reactive fluorescent probe, meso-DichlorotriazineEthyl BODIPY (mDTEB), tailor-made for labeling CNFs used in simulated or in vivo ingestion exposure studies. Time-correlated single photon counting (TCSPC) fluorescence lifetime imaging microscopy (FLIM) was used to confirm covalent attachment and purity of mDTEB-labeled CNFs. The photoluminescence properties of mDTEB-labeled CNFs, characterized using fluorescence spectroscopy, include excellent stability over a wide pH range (pH2 to pH10) and high quantum yield, which provides detection at low (μM) concentrations. FLIM analysis also showed that lignin-like impurities present on the CNF reduce the fluorescence of the mDTEB-labeled CNF, via quenching. Therefore, the chemical composition and the methods of CNF production affect subsequent studies. An in vitro triculture, small intestinal, epithelial model was used to assess the toxicity of ingested mDTEB-labeled CNFs. Zebrafish (Danio rerio) were used to assess in vivo environmental toxicity studies. No cytotoxicity was observed for CNFs, or mDTEB-labeled CNFs, either in the triculture cells or in the zebrafish embryos.