Volumetric Temperature Mapping Using Light-Sheet Microscopy and Upconversion Fluorescence from Micro- and Nano-Rare Earth Composites

Authors:
Open Access
Publisher website
Google Scholar
Abstract
We present a combination of light-sheet excitation and two-dimensional fluorescence intensity ratio (FIR) measurements as a simple and promising technique for three-dimensional temperature mapping. The feasibility of this approach is demonstrated with samples fabricated with sodium yttrium fluoride nanoparticles co-doped with rare-earth ytterbium and erbium ions (NaYF4:Yb3+/Er3+) incorporated into polydimethylsiloxane (PDMS) as a host material. In addition, we also evaluate the technique using lipid-coated NaYF4:Yb3+/Er3+ nanoparticles immersed in agar. The composite materials show upconverted (UC) fluorescence bands when excited by a 980 nm near-infrared laser light-sheet. Using a single CMOS camera and a pair of interferometric optical filters to specifically image the two thermally-coupled bands (at 525 and 550 nm), the two-dimensional FIR and, hence, the temperature map can be readily obtained. The proposed method can take optically sectioned (confocal-like) images with good optical resolution over relatively large samples (up to the millimetric scale) for further 3D temperature reconstruction.
Funding Information
  • Secretaría de Ciencia, Humanidades, Tecnología e Innovación (317184, 246648, 871124, CEX2019-000910-S [MCIN/ AEI/10.13039/501100011033], PID2021-122807OB-C31)
  • Secretaría de Ciencia, Humanidades, Tecnología e Innovación (317184, 246648, 871124, CEX2019-000910-S [MCIN/ AEI/10.13039/501100011033], PID2021-122807OB-C31)
  • Laserlab-Europe (317184, 246648, 871124, CEX2019-000910-S [MCIN/ AEI/10.13039/501100011033], PID2021-122807OB-C31)
  • Fundació Cellex, Fundació Mir-Puig, Generalitat de Catalunya through CERCA (317184, 246648, 871124, CEX2019-000910-S [MCIN/ AEI/10.13039/501100011033], PID2021-122807OB-C31)
  • Digital Retina project (317184, 246648, 871124, CEX2019-000910-S [MCIN/ AEI/10.13039/501100011033], PID2021-122807OB-C31)

This publication has 47 references indexed in Scilit: