Encoding of whisker input by cerebellar Purkinje cells

Authors:
  • SKSebastiaan K. E. Koekkoek
    Sebastiaan K. E. Koekkoek
  • JSJoël Shapiro
    Joël Shapiro
  • BRBianca F. M. Rijken
    Bianca F. M. Rijken
  • FZFroukje Zandstra
    Froukje Zandstra
  • BEBarry Van Der Ende
    Barry Van Der Ende
  • COCullen B. Owens
    Cullen B. Owens
  • JPJan-Willem Potters
    Jan-Willem Potters
  • JGJornt R. De Gruijl
    Jornt R. De Gruijl
  • TRTom J. H. Ruigrok
    Tom J. H. Ruigrok
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Abstract
The cerebellar cortex is crucial for sensorimotor integration. Sensorimotor inputs converge on cerebellar Purkinje cells via two afferent pathways: the climbing fibre pathway triggering complex spikes, and the mossy fibre–parallel fibre pathway, modulating the simple spike activities of Purkinje cells. We used, for the first time, the mouse whisker system as a model system to study the encoding of somatosensory input by Purkinje cells.We show that most Purkinje cells in ipsilateral crus 1 and crus 2 of awake mice respond to whisker stimulation with complex spike and/or simple spike responses. Single-whisker stimulation in anaesthetised mice revealed that the receptive fields of complex spike and simple spike responses were strikingly different. Complex spike responses, which proved to be sensitive to the amplitude, speed and direction of whisker movement, were evoked by only one or a few whiskers. Simple spike responses, which were not affected by the direction of movement, could be evoked by many individual whiskers. The receptive fields of Purkinje cells were largely intermingled, and we suggest that this facilitates the rapid integration of sensory inputs from different sources. Furthermore, we describe that individual Purkinje cells, at least under anaesthesia, may be bound in two functional ensembles based on the receptive fields and the synchrony of the complex spike and simple spike responses. The ‘complex spike ensembles’ were oriented in the sagittal plane, following the anatomical organization of the climbing fibres, while the ‘simple spike ensembles’ were oriented in the transversal plane, as are the beams of parallel fibres.

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