The genus Lasioglossum Curtis, 1833 is the most diverse group of halictid bees and is widely distributed across the Palaearctic region. In the Mediterranean Basin, a recognized biodiversity hotspot, Lasioglossum is especially well represented, yet its taxonomy remains complex and often unresolved. This is particularly true in Italy, where the fauna of the Western part of the Mediterranean Basin is mixing with the Eastern part. Moreover, previous taxonomic studies on the species recorded in Italy are sporadic, scattered across different sources, and often published in languages other than Italian. As a result, identification tools remain fragmented and largely inaccessible to a broader audience. Through a thorough examination of both historical and recent material, together with a critical review of the relevant taxonomic literature, this study provides the first comprehensive revision of the Italian Lasioglossum fauna. 103 species are now recognised from Italy, including one taxon returned to species rank from its previous subspecific combination: Lasioglossum pseudocombinatum (Blüthgen, 1921) stat. rev. Thirteen taxa previously cited in the literature are here excluded or revised in their taxonomic status. Italy ranks as the third most species-rich country in Europe for Lasioglossum, following Greece and Spain. Despite this richness, the level of strict endemism is low (0.94%), confirming Italy’s complex biogeographic setting at the intersection of major faunal regions. The current assemblage is shaped more by colonisation and faunal exchange than by isolation or speciation. This study also includes the first complete identification key to all Italian species. By consolidating scattered data and providing accessible tools for species identification, it aims to promote further research on wild bee diversity in Italy and support conservation efforts in a context where taxonomic knowledge has long remained fragmentary.