Immobilization of Peroxidase onto Magnetite Modified Polyaniline
Open Access
- 1 January 2012
- journal article
- Published by Hindawi Limited in The Scientific World Journal
- Vol. 2012, 1-5
Abstract
The present study describes the immobilization of horseradish peroxidase (HRP) on magnetite-modified polyaniline (PANImG) activated with glutaraldehyde. After the optimization of the methodology, the immobilization of HRP on PANImG produced the same yield (25%) obtained for PANIG with an efficiency of 100% (active protein). The optimum pH for immobilization was displaced by the effect of the partition of protons produced in the microenvironment by the magnetite. The tests of repeated use have shown that PANImG-HRP can be used for 13 cycles with maintenance of 50% of the initial activity.Keywords
Funding Information
- Fundación Carolina
This publication has 25 references indexed in Scilit:
- The immobilization of trypsin onto polyaniline for protein digestionMaterials Science and Engineering: C, 2009
- A hydrogen peroxide biosensor based on polyaniline/FTOSensors and Actuators B: Chemical, 2009
- Enhancing the sensitivity and stability of HRP/PANI/Pt electrode by implanted bovine serum albuminBiosensors and Bioelectronics, 2008
- Ordered mesoporous polyaniline film as a new matrix for enzyme immobilization and biosensor constructionAnalytica Chimica Acta, 2007
- Enhancement of a conducting polymer-based biosensor using carbon nanotube-doped polyanilineAnalytica Chimica Acta, 2006
- Immobilization of Aspergillus niger glucoamylase onto a polyaniline polymerProcess Biochemistry, 2005
- Electrochemistry and scanning electron microscopy of polyaniline/peroxidase-based biosensorTalanta, 2004
- Properties of horseradish peroxidase immobilised onto polyanilineProcess Biochemistry, 2004
- Immobilization of horseradish peroxidase onto polyaniline polymersProcess Biochemistry, 2003
- Bioelectrochemical responses of the polyaniline horseradish peroxidase electrodesJournal of Electroanalytical Chemistry, 1997