Rhodopsin Forms a Dimer with Cytoplasmic Helix 8 Contacts in Native Membranes
- 18 February 2012
- journal article
- other
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 51 (9) , 1819-1821
Abstract
G protein-coupled receptors form dimers and higher-order oligomers in membranes, but the precise mode of receptor–receptor interaction remains unknown. To probe the intradimeric proximity of helix 8 (H8), we conducted chemical cross-linking of endogenous cysteines in rhodopsin in disk membranes. We identified a Cys316–Cys316 cross-link using partial proteolysis and liquid chromatography with mass spectrometry. These results show that a symmetric dimer interface mediated by H1 and H8 contacts is present in native membranes.Keywords
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