p16 Gene Methylation in Colorectal Tumors: Correlation with Clinicopathological Features and Prognostic Value
Authors:
- M.L. MaestroMMM.L. Maestro
- M. VidaurretaMVM. Vidaurreta
- C. MaestroCMC. Maestro
- M. ArroyoMAM. Arroyo
- J. CerdánJCJ. Cerdán
- 1 December 2005
- journal article
- research article
- Published by S. Karger AG in Digestive Diseases
- Vol. 23 (2) , 151-155
Abstract
Background: Methylation of the CpG islands in the p16 gene promoter region is an important transcription repression mechanism that has been identified as an alternative mechanism for inactivating specific genes in cancer. Given that, DNA methylation is a common event in colorectal cancer. Materials and Methods: The aim of this study was to establish the methylation status of the p16 gene in 104 patients with colorectal carcinoma and evaluate its prognostic value. DNA was bisulfite-modified and analyzed for p16 promoter methylation by methylation-specific PCR. Results: Methylation of thep16 gene was determined in 18.3% of our patient population (19/104). The methylated state did not correlate with any clinicopathological factors. During a median follow-up period of 72 months, the overall survival rate for patients with gene methylation was 75% and without gene methylation it was 61% (p = 0.09). Conclusions: Although not statistically significant, this difference indicates a clinically valuable tendency.Keywords
This publication has 17 references indexed in Scilit:
- Recidiva locorregional en el cáncer de rectoCirugía Española, 2003
- Prognostic significance of extensive microsatellite instability in sporadic clinicopathological stage C colorectal cancerBritish Journal of Surgery, 2000
- Tumor Microsatellite Instability and Clinical Outcome in Young Patients with Colorectal CancerNew England Journal of Medicine, 2000
- Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.Proceedings of the National Academy of Sciences, 1996
- 5′ CpG island methylation is associated with transcriptional silencing of the tumour suppressor p16/CDKN2/MTS1 in human cancersNature Medicine, 1995
- Deletions of the cyclin-dependent kinase-4 inhibitor gene in multiple human cancersNature, 1994
- A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4Nature, 1993
- Ubiquitous somatic mutations in simple repeated sequences reveal a new mechanism for colonic carcinogenesisNature, 1993
- The essentials of DNA methylationCell, 1992
- A genetic model for colorectal tumorigenesisCell, 1990