Interaction between troponin I and troponin C
Authors:
- 13 December 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 150 (1) , 54-58
Abstract
The spatial proximity on the surface of troponin C for sites specific for peptides CN4 (res. 96–116) and CN5 (res. 1–21) of troponin I has been demonstrated by proton magnetic resonance spectroscopy. The broadened signals from each peptide are at similar radial distances from the paramagnetic spin label bound to Cys 98 of troponin C. A U‐shaped disposition of peptide CN5 about Cys 98 is indicated, the interaction being modulated by calcium binding to the low affinity Ca2+ domains of troponin C. Paramagnetic broadening of signals from peptide CN4 was observed on addition of peptide TR2 (res. 88–159) from spin‐labelled troponin C, a region that contains the higher affinity Ca2+ domains.Keywords
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