Biochemical Characterization of CPS-1, a Subclass B3 Metallo-β-Lactamase from a Chryseobacterium piscium Soil Isolate
Open Access
- 1 March 2016
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 60 (3) , 1869-1873
Abstract
CPS-1 is a subclass B3 metallo-β-lactamase from a Chryseobacterium piscium isolated from soil, showing 68 % amino acid identity to GOB-1 enzyme. CPS-1 was overproduced in Escherichia coli Rosetta (DE3), purified by chromatography and biochemically characterized. This enzyme exhibits a broad spectrum substrate profile including penicillins, cephalosporins and carbapenems, which overall resembles those of L1, GOB-1 and acquired subclass B3 enzymes AIM-1 and SMB-1.Keywords
This publication has 28 references indexed in Scilit:
- Genetic and Biochemical Characterization of an Acquired Subgroup B3 Metallo-β-Lactamase Gene, bla AIM-1 , and Its Unique Genetic Context in Pseudomonas aeruginosa from AustraliaAntimicrobial Agents and Chemotherapy, 2012
- Crystal Structure of the Mobile Metallo-β-Lactamase AIM-1 from Pseudomonas aeruginosa: Insights into Antibiotic Binding and the Role of Gln157Antimicrobial Agents and Chemotherapy, 2012
- In VivoImpact of Met221 Substitution in GOB Metallo-β-LactamaseAntimicrobial Agents and Chemotherapy, 2012
- SMB-1, a Novel Subclass B3 Metallo-β-Lactamase, Associated with IS CR1 and a Class 1 Integron, from a Carbapenem-Resistant Serratia marcescens Clinical IsolateAntimicrobial Agents and Chemotherapy, 2011
- IND-6, a Highly Divergent IND-Type Metallo-β-Lactamase fromChryseobacterium indologenesStrain 597 Isolated in Burkina FasoAntimicrobial Agents and Chemotherapy, 2009
- Carbapenemases: the Versatile β-LactamasesClinical Microbiology Reviews, 2007
- Postgenomic Scan of Metallo-β-Lactamase Homologues in Rhizobacteria: Identification and Characterization of BJP-1, a Subclass B3 Ortholog fromBradyrhizobium japonicumAntimicrobial Agents and Chemotherapy, 2006
- Genetic and Biochemical Characterization of CGB-1, an Ambler Class B Carbapenem-Hydrolyzing β-Lactamase from Chryseobacterium gleumAntimicrobial Agents and Chemotherapy, 2002
- Biochemical Characterization of the FEZ-1 Metallo-β-Lactamase of Legionella gormanii ATCC 33297 T Produced in Escherichia coliAntimicrobial Agents and Chemotherapy, 2001
- Standard Numbering Scheme for Class B β-LactamasesAntimicrobial Agents and Chemotherapy, 2001