RNA degrading activity is tightly associated with the multicatalytic proteinase, ingensin
Authors:
- Ken-Ichi TanakaKTKen-Ichi Tanaka
- Toshio OgawaTOToshio Ogawa
- Shoichi IshiuraSIShoichi Ishiura
- Ryuhei FunabikiRFRyuhei Funabiki
- Hideo SugitaHSHideo Sugita
- 11 September 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 255 (1) , 179-183
Abstract
The multicatalytic proteinase, ingensin, was purified to homogeneity from chicken liver. rRNA‐degrading activity was co‐eluted with the purified multicatalytic proteinase from a TSK‐3000SW column. This RNA‐degrading activity was inactivated by heat treatment and the addition of a low concentration of SDS. Therefore, the RNA‐degrading activity co‐eluted with the multicatalytic proteinase was not due to contamination by low‐molecular‐mass RNases. These results strongly suggest that this RNA‐degrading activity was tightly associated with the multicatalytic proteinase, ingensin.Keywords
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