Regulation of the Biological Functions of Osteoblasts and Bone Formation by Zn-Incorporated Coating on Microrough Titanium

Authors:
Publisher website
Google Scholar
Abstract
To improve the biological performance of titanium implant, a series of Zn-incorporated coatings were fabricated on the microrough titanium (Micro-Ti) via sol–gel method by spin-coating technique. The successful fabrication of the coating was verified by combined techniques of scanning electron microscopy, surface profiler, X-ray diffraction, X-ray photoelectron spectroscopy, and water contact angle measurements. The incorporated zinc existed as ZnO, which released Zn ions in a sustained manner. The Zn-incorporated samples (Ti–Zn0.08, Ti–Zn0.16, and Ti–Zn0.24) efficiently inhibited the adhesion of both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria. The in vitro evaluations including cell activity, alkaline phosphatase (ALP), mineralization, osteogenic genes expressions (Runx2, ALP, OPG, Col I, OPN, and OC), and tartrate-resistant acid phosphatase, confirmed that Ti–Zn0.16 sample was the optimal one to regulate the proliferation or differentiation for both osteoblasts and osteoclasts. More importantly, in vivo evaluations including Micro-CT analysis, push-out test, and histological observations verified that Ti–Zn0.16 implants could efficiently promote new bone formation after implantation for 4 and 12 weeks, respectively. The resulting material thus has potential application in orthopedic field.
Funding Information
  • Ministry of Education of the People's Republic of China (CQDXWL-2013-Z002)
  • Ministry of Science and Technology of the People's Republic of China (2012BAI18B04)
  • National Natural Science Foundation of China (31200712, 51173216)
  • Natural Science Foundation of Chongqing City (2011JJJQ10004)

This publication has 57 references indexed in Scilit: