Effect of Intracanal Medications on the Viability of Human Periodontal Ligament‐Derived Mesenchymal Stem Cells

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Abstract
This in vitro study evaluated the effects of intracanal medications on the metabolic activity and adhesion of human periodontal ligament‐derived mesenchymal stem cells. Tested groups included Control (without medication), Triple Antibiotic Paste (TAP), Double Antibiotic Paste (DAP), calcium hydroxide with saline, calcium hydroxide with 2% chlorhexidine gel and Bio‐C Temp. After conditioning, roots were stored in phosphate‐buffered saline at 37°C for 21 days. Medications were then removed using 17% EDTA and saline, and dentine samples were used as substrates for cell culture at 3, 5 and 7 days. Metabolic activity was assessed via MTT assay, and adhesion via scanning electron microscopy. None of the medications negatively affected cell viability or adhesion compared to control. TAP showed higher metabolic activity than calcium hydroxide with saline at Day 7. DAP induced earlier cell morphology changes. Bio‐C Temp and chlorhexidine gel showed no significant differences at Day 7, confirming overall biocompatibility.
Funding Information
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (001)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (303587/2022‐9)
  • Fundo de Apoio ao Ensino, à Pesquisa e Extensão, Universidade Estadual de Campinas

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