Reverse Phase Thin Layer Chromatography Assay of Vitamin K in Bovine Liver
Authors:
- 1 September 1993
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 16 (13) , 2825-2834
Abstract
A case of hemorrhage of unknown origin was observed in cattle and liver samples were submitted to the Diagnostic Lab for assay of Vitamin K or possible coumarol. After evaluating normal phase and reverse phase Thin Layer Chromatography (TLC) plates and different solvents, reverse phase TLC plate with indicator and methylene chloride:methanol 70:30 were chosen for direct method of detection and indication of Vitamin K. Gas Chromatography and Densitometry were used to guantitate the Vitamin K present in liver samples and confirmation of Vitamin K was done by Mass Spectroscopy.Keywords
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