Separation of Large Quantities of Ribosomal Subunits by Zonal Ultracentrifugation
Authors:
Open Access
- 1 January 1970
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 12 (1) , 113-116
Abstract
The maximum capacity of the B‐XV zonal rotor for the separation of Escherichia coli ribosomal subunits has been investigated. These experiments were performed using a class of sucrose density gradients (hyperbolic gradients) which present certain theoretical advantages. 2 g of subunits could be utilised in single centrifugations with a final yield of 0.5 g of 30 S and 1.0 g of 50 S subunits of greater than 99° purity.Keywords
This publication has 7 references indexed in Scilit:
- Sedimentation rates of plastids in an analytical zonal rotorBiochimica et Biophysica Acta (BBA) - General Subjects, 1967
- The capacity of zones in density-gardient centrifugationBiochimica et Biophysica Acta (BBA) - General Subjects, 1967
- Ribosomal proteins of Escherichia coli. I. Demonstration of different primary structures.Proceedings of the National Academy of Sciences, 1967
- Zonal Ultracentrifuge for the Separation of Ribosomal SubunitsScience, 1966
- THE ZONAL ULTRACENTRIFUGE. A NEW INSTRUMENT FOR FRACTIONATING MIXTURES OF PARTICLESThe Journal of Physical Chemistry, 1962
- High-Resolution Density Gradient Sedimentation AnalysisScience, 1960
- Ribonucleoprotein particles from Escherichia coliJournal of Molecular Biology, 1959