Elimination of Foreign Sequences in Eukaryotic Viral Reference Genomes Improves the Accuracy of Virome Analysis
Open Access
- 20 December 2022
- journal article
- research article
- Published by American Society for Microbiology in mSystems
- Vol. 7 (6) , e0090722
Abstract
High-throughput sequencing-based viromics highly depends on reference databases, but foreign contamination is widespread in public databases and often leads to confusing and even wrong conclusions in genomic analysis and viromic profiling. To address this issue, we systematically detected and identified the contamination in the largest viral sequence collections of GenBank and UniProt based on a stringent scrutiny pipeline.Keywords
Funding Information
- MOST | National Key Research and Development Program of China (2021YFD1801101)
- National Natural Science Foundation of China (32022083)
- National Natural Science Foundation of China (32130104)
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