[Development of diagnostic kit "Ptakh-Grip PLR" for detection of highly pathogenic strain of avian influenza virus H5N1 by polymerase chain reaction].

Authors:
  • IBI Iu Bisiuk
    I Iu Bisiuk
  • SMS D Mel'Nichuk
    S D Mel'Nichuk
  • VSV G Spiridonov
    V G Spiridonov
  • DMD L Martynenko
    D L Martynenko
  • MMM D Mel'Nichuk
    M D Mel'Nichuk
  • VLV T Lisovenko
    V T Lisovenko

  • 15 November 2006
    • journal article
    • abstracts
Abstract
A diagnostic kit for detection of avian influenza virus by real-time polymerase chain reaction was developed. This kit allows to identify the influenza viruses type A and highly pathogenic strain of avian influenza virus H5N1. The diagnostic kit is universal and adopted for ABI PRISM SDS (Applied Biosystems), RotorGene (Corbett Research) and iQCycler (BioRad) PCR machines.