Genotoxicity of Environmentally Relevant Concentrations of Water-Soluble Oil Components in Cod (Gadus morhua)
Authors:
- 3 April 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Environmental Science & Technology
- Vol. 43 (9) , 3329-3334
Abstract
Large discharges from oil and gas production platforms (produced water) have led to concerns for adverse biological effects in marine areas. The aim of this study was to investigate the development of DNA adduct formation and related biomarkers in fish after chronic exposure to water-soluble components of oil. Atlantic cod (Gadus morhua) were exposed for up to 44 weeks to three treatments (low, pulsed, high) containing environmentally relevant concentrations of low-molecular-weight polycyclic aromatic hydrocarbons (PAHs) and short-chained alkylphenols (APs). A time- and dose-related pattern of DNA adduct formation (measured using 32P-postlabeling) was observed. The results suggested that an extended exposure period (more than 16 weeks) would be required for the formation of DNA adduct levels above background. Interestingly, fish receiving pulsed high exposure did not develop elevated concentrations of DNA adducts, possibly due to DNA repair processes. No obvious relationship between DNA adduct concentration and cytochrome P4501A activity (EROD) was observed. This study has demonstrated the genotoxic potential of water-soluble oil components, relevant for operational discharges (produced water) and chronic oil spills. The quantification of PAH metabolites in bile and hepatic DNA adduct formation appear to be suitable for environmental monitoring of chronic oil pollution.Keywords
This publication has 41 references indexed in Scilit:
- DNA adducts in fish following an oil spill exposureEnvironmental Chemistry Letters, 2006
- May Organic Pollutants Affect Fish Populations in the North Sea?Journal of Toxicology and Environmental Health, Part A, 2006
- Establishing the Causal Relationship between Polycyclic Aromatic Hydrocarbon (PAH) Exposure and Hepatic Neoplasms and Neoplasia-Related Liver Lesions in English Sole (Pleuronectes vetulus)Human and Ecological Risk Assessment: An International Journal, 2003
- Evidence of uptake, biotransformation and DNA binding of polyaromatic hydrocarbons in Atlantic cod and corkwing wrasse caught in the vicinity of an aluminium worksMarine Environmental Research, 2001
- PAH metabolites in bile, cytochrome P4501A and DNA adducts as environmental risk parameters for chronic oil exposure: a laboratory experiment with Atlantic codAquatic Toxicology, 2000
- DNA adduct formation in northern pike (Esox lucius) exposed to a mixture of benzo[a]pyrene, benzo[k]fluoranthene and 7H-dibenzo[c,g]carbazole: time-course and dose-response studiesMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2000
- Fixed wavelength fluorescence (FF) of bile as a monitoring tool for polyaromatic hydrocarbon exposure in fish: an evaluation of compound specificity, inner filter effect and signal interpretationBiomarkers, 2000
- Molecular epizootiology of genotoxic events in marine fish: Linking contaminant exposure, DNA damage, and tissue-level alterationsMutation Research - Reviews in Mutation Research, 1998
- The cytochrome P-450 system in fish, aquatic toxicology and environmental monitoringAquatic Toxicology, 1992
- The uptake and distribution of [3H]benzo[a]pyrene in the Northern pike (Esox lucius). Examination by whole-body autoradiography and scintillation countingToxicology and Applied Pharmacology, 1984