Analysis of the Human Dopamine β‐Hydroxylase Promoter: Transcriptional Induction by Cyclic AMP

Authors:
Publisher website
Google Scholar
Abstract
We have analyzed some functional aspects of the promoter of the human dopamine β‐hydroxylase (DBH) gene. A fragment of 1,247 bp directly 5′ to the transcriptional start was progressively shortened, placed in front of a reporter gene, and tested in a human neuroblastoma cell line expressing DBH (SK‐N‐SH‐TFM) and in a monkey kidney cell line (CV‐1). A remarkably short region (267 bp), directly upstream from the transcription start, was sufficient to confer activity and tissue‐specific expression. Furthermore, the expression of the DBH gene was shown to be inducible by cyclic AMP in SK‐N‐SH‐TFM cells. This effect was demonstrated to occur at the transcriptional level, as shown by run‐on assays, and was due to the presence of a near‐consensus cyclic AMP‐responsive element located in the untranscribed 5′ regulatory region of the gene.

This publication has 19 references indexed in Scilit: