Packaging of DNA origami in viral capsids
- 17 April 2019
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Nanoscale
- Vol. 11 (21) , 10160-10166
Abstract
Here we show the encapsulation of 35 nm diameter, nearly-spherical, DNA origami by self-assembly of SV40-like (simian virus 40) particles. The self-assembly of this new type of nanoparticles is highly reproducible and efficient. The structure of these particles was determined by cryo-EM. The capsid forms a regular SV40 lattice of T = 7d icosahedral symmetry and the structural features of encapsulated DNA origami are fully visible. These particles are a promising biomaterial for use in various medical applications.Keywords
Funding Information
- Ministry of Science and Technology, Israel
- Horizon 2020 Framework Programme (744184)
- H2020 Marie Skłodowska-Curie Actions (708572)
This publication has 35 references indexed in Scilit:
- Viral genome structures are optimal for capsid assemblyeLife, 2013
- To Build a Virus on a Nucleic Acid SubstrateBiophysical Journal, 2013
- Effect of capsid confinement on the chromatin organization of the SV40 minichromosomeNucleic Acids Research, 2012
- Differential assembly of Hepatitis B Virus core protein on single- and double-stranded nucleic acid suggest the dsDNA-filled core is spring-loadedVirology, 2012
- RNA Encapsidation by SV40-Derived Nanoparticles Follows a Rapid Two-State MechanismJournal of the American Chemical Society, 2012
- The structure of avian polyomavirus reveals variably sized capsids, non-conserved inter-capsomere interactions, and a possible location of the minor capsid protein VP4Virology, 2011
- Role of Surface Charge Density in Nanoparticle-Templated Assembly of Bromovirus Protein CagesACS Nano, 2010
- Self-assembly approaches to nanomaterial encapsulation in viral protein cagesJournal of Materials Chemistry, 2008
- Core-controlled polymorphism in virus-like particlesProceedings of the National Academy of Sciences, 2007
- The structure of simian virus 40 refined at 3.1 å resolutionStructure, 1996