Reversible Energy-Transfer Switching on a DNA Scaffold
Open Access
- 17 February 2015
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 137 (7) , 2444-2447
Abstract
We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.Keywords
Funding Information
- Biotechnology and Biological Sciences Research Council (BB/J001694/1)
- European Research Council (203952)
- Vetenskapsrådet (622-2010-280)
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