Growth Inhibition and Apoptosis of Neuroblastoma Cells Through ROS-Independent MEK/ERK Activation by Sulforaphane
- 16 February 2013
- journal article
- research article
- Published by Springer Nature in Cell Biochemistry and Biophysics
- Vol. 66 (3) , 765-774
Abstract
Deregulation of apoptosis alters the balance of cell proliferation and cell death, resulting in a variety of diseases, including cancer. In recent studies, sulforaphane (SFN) has demonstrated potent anti-tumor and chemopreventive activities. A possible signal transduction pathway has also been elucidated for SFN-induced apoptosis in human neuroblastoma SH-SY5Y cells. The present study further investigates the anti-proliferation activities of SFN through induced apoptosis in SH-SY5Y cells. We found that treating SH-SY5Y cells with SFN resulted in the depletion of mitochondrial membrane potential (Δψ), which in turn increased caspase 9, caspase 3, and the up-regulation of phosphorylated MEK/ERK without generating reactive oxygen species. Results were confirmed by MTT assay, which demonstrated the cytotoxic activity of SFN against SH-SY5Y cells (IC50 values of 20 μM).Keywords
This publication has 34 references indexed in Scilit:
- Sulforaphane protects SK‐N‐SH cells against antipsychotic‐induced oxidative stressFundamental & Clinical Pharmacology, 2011
- Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by SulforaphaneEvidence-Based Complementary and Alternative Medicine, 2011
- Anti-tumor activity and signaling events triggered by the isothiocyanates, sulforaphane and phenethyl isothiocyanate, in multiple myelomaHaematologica, 2011
- Anti-angiogenic effects of dietary isothiocyanates: Mechanisms of action and implications for human healthBiochemical Pharmacology, 2011
- Curcuminoids Suppress the Growth and Induce Apoptosis through Caspase-3-Dependent Pathways in Glioblastoma Multiforme (GBM) 8401 CellsJournal of Agricultural and Food Chemistry, 2010
- Sensitization of Non-small Cell Lung Cancer Cells to Cisplatin by Naturally Occurring IsothiocyanatesChemical Research in Toxicology, 2010
- Mouse models for unraveling the importance of diet in colon cancer preventionThe Journal of Nutritional Biochemistry, 2010
- Allyl isothiocyanate as a cancer chemopreventive phytochemicalMolecular Nutrition & Food Research, 2010
- Sulforaphane as an inducer of glutathione prevents oxidative stress‐induced cell death in a dopaminergic‐like neuroblastoma cell lineJournal of Neurochemistry, 2009
- Role of Phase II Drug Metabolizing Enzymes in Cancer ChemopreventionCurrent Drug Metabolism, 2009