Structure and dynamics of retrotransposons at wheat centromeres and pericentromeres
- 22 May 2008
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 117 (5) , 445-456
Abstract
Little is known of the dynamics of centromeric DNA in polyploid plants. We report the sequences of two centromere-associated bacterial artificial chromosome clones from a Triticum boeoticum library. Both autonomous and non-autonomous wheat centromeric retrotransposons (CRWs) were identified, both being closely associated with the centromeres of wheat. Fiber-fluorescence in situ hybridization and chromatin immunoprecipitation analysis showed that wheat centromeric retrotransposons (CRWs) represent a dominant component of the wheat centromere and are associated with centromere function. CRW copy number showed variation among different genomes: the D genome chromosomes contained fewer copies than either the A or B genome chromosomes. The frequency of lengthy continuous CRW arrays was higher than that in either rice or maize. The dynamics of CRWs and other retrotransposons at centromeric and pericentromeric regions during diploid speciation and polyploidization of wheat and its related species are discussed.Keywords
This publication has 55 references indexed in Scilit:
- Evolutionary History and Positional Shift of a Rice CentromereGenetics, 2007
- Ecotype-specific and chromosome-specific expansion of variant centromeric satellites in Arabidopsis thalianaMolecular Genetics and Genomics, 2006
- Genomic and Genetic Characterization of RiceCen3Reveals Extensive Transcription and Evolutionary Implications of a Complex CentromereThe Plant Cell, 2006
- Identification and Preliminary Analysis of Several Centromere‐associated Bacterial Artificial Chromosome Clones from a Diploid Wheat LibraryJournal of Integrative Plant Biology, 2006
- Characterization of CENH3 and centromere-associated DNA sequences in sugarcaneChromosome Research, 2005
- A direct repeat sequence associated with the centromeric retrotransposons in wheatGenome, 2004
- Functional Rice Centromeres Are Marked by a Satellite Repeat and a Centromere-Specific RetrotransposonThe Plant Cell, 2002
- Transposable elements, genes and recombination in a 215-kb contig from wheat chromosome 5AmFunctional & Integrative Genomics, 2002
- Application of fiber-FISH in physical mapping of Arabidopsis thalianaGenome, 1998
- Substitution rate comparisons between grasses and palms: synonymous rate differences at the nuclear gene Adh parallel rate differences at the plastid gene rbcL.Proceedings of the National Academy of Sciences, 1996