Gut Microbiome Dysbiosis in Patients with Pemphigus and Correlation with Pathogenic Autoantibodies

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Abstract
Background: Pemphigus is a group of potentially life-threatening autoimmune bullous diseases induced by pathogenic autoantibodies binding to the surface of epidermal cells. The role of the gut microbiota (GM) has been described in various autoimmune diseases. However, the impact of the GM on pemphigus is less understood. This study aimed to investigate whether there was alterations in the composition and function of the GM in pemphigus patients compared to healthy controls (HCs). Methods: Fecal samples were collected from 20 patients with active pemphigus (AP), 11 patients with remission pemphigus (PR), and 47 HCs. To sequence the fecal samples, 16S rRNA was applied, and bioinformatic analyses were performed. Results: We found differences in the abundance of certain bacterial taxa among the three groups. At the family level, the abundance of Prevotellaceae and Coriobacteriaceae positively correlated with pathogenic autoantibodies. At the genus level, the abundance of Klebsiella, Akkermansia, Bifidobacterium, Collinsella, Gemmiger, and Prevotella positively correlated with pathogenic autoantibodies. Meanwhile, the abundance of Veillonella and Clostridium_XlVa negatively correlated with pathogenic autoantibodies. A BugBase analysis revealed that the sum of potentially pathogenic bacteria was elevated in the AP group in comparison to the PR group. Additionally, the proportion of Gram-negative bacteria in the PR group was statistically significantly lower in comparison to the HC group. Conclusion: The differences in GM composition among the three groups, and the correlation between certain bacterial taxa and pathogenic autoantibodies of pemphigus, support a linkage between the GM and pemphigus.
Funding Information
  • Natural Science Foundation of Beijing Municipality (7232118, 2022-PUMCH-B-092, 2022-PUMCH-A-026, 82270405, 20220484031)
  • National High Level Hospital Clinical Research Funding (7232118, 2022-PUMCH-B-092, 2022-PUMCH-A-026, 82270405, 20220484031)
  • National Natural Science Foundation of China (7232118, 2022-PUMCH-B-092, 2022-PUMCH-A-026, 82270405, 20220484031)
  • Beijing Nova Program (7232118, 2022-PUMCH-B-092, 2022-PUMCH-A-026, 82270405, 20220484031)
  • National Key Clinical Specialty Project of China (7232118, 2022-PUMCH-B-092, 2022-PUMCH-A-026, 82270405, 20220484031)

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