Label-free methods for optical in vitro characterization of protein–protein interactions

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Abstract
Protein–protein interactions are involved in the regulation and function of the majority of cellular processes. As a result, much effort has been aimed at the development of methodologies capable of quantifying protein–protein interactions, with label-free methods being of particular interest due to the associated simplified workflows and minimisation of label-induced perturbations. Here, we review recent advances in optical technologies providing label-free in vitro measurements of affinities and kinetics. We provide an overview and comparison of existing techniques and their principles, discussing advantages, limitations, and recent applications.
Funding Information
  • H2020 European Research Council (PHOTOMASS 819593)
  • Engineering and Physical Sciences Research Council (EP/T03419X/1)
  • Medical Research Council (MR/V02213X/1)

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