JAM-1 is a ligand of the β2 integrin LFA-1 involved in transendothelial migration of leukocytes
Authors:
- 14 January 2002
- journal article
- research article
- Published by Springer Nature in Nature Immunology
- Vol. 3 (2) , 151-158
Abstract
Inflammatory recruitment of leukocytes is governed by dynamic interactions between integrins and endothelial immunoglobulin superfamily (IgSF) proteins. We have identified the IgSF member junctional adhesion molecule 1 (JAM-1) as a ligand of the β2 integrin lymphocyte function–associated antigen 1 (LFA-1). Under static and physiological flow conditions, JAM-1 contributed to LFA-1–dependent transendothelial migration of T cells and neutrophils as well as LFA-1–mediated arrest of T cells. The latter was triggered by chemokines on endothelium that was stimulated with cytokines to redistribute JAM-1 from the tight junctions. Transfectants expressing JAM-1 supported LFA-1–mediated adhesion of leukocytes, which required the membrane-proximal Ig-like domain 2 of JAM-1. Thus, JAM-1 is a counter-receptor for LFA-1 that is ideally situated to guide and control transmigration during leukocyte recruitment.Keywords
This publication has 49 references indexed in Scilit:
- A Novel Protein with Homology to the Junctional Adhesion MoleculeJournal of Biological Chemistry, 2000
- Junctional Adhesion Molecule (JAM) Is Phosphorylated by Protein Kinase C upon Platelet ActivationBiochemical and Biophysical Research Communications, 2000
- Junctional Adhesion Molecule Interacts with the PDZ Domain-containing Proteins AF-6 and ZO-1Journal of Biological Chemistry, 2000
- Interaction of Junctional Adhesion Molecule with the Tight Junction Components ZO-1, Cingulin, and OccludinJournal of Biological Chemistry, 2000
- Localization of the Binding Site on Intercellular Adhesion Molecule-3 (ICAM-3) for Lymphocyte Function-associated Antigen 1 (LFA-1)Journal of Biological Chemistry, 1996
- Sequential regulation of alpha 4 beta 1 and alpha 5 beta 1 integrin avidity by CC chemokines in monocytes: implications for transendothelial chemotaxis.Journal of Cell Biology, 1996
- Traffic signals for lymphocyte recirculation and leukocyte emigration: The multistep paradigmCell, 1994
- Intercellular adhesion molecule 3, a third adhesion counter-receptor for lymphocyte function-associated molecule 1 on resting lymphocytes.Journal of Experimental Medicine, 1992
- Binding of the integrin Mac-1 (CD11b/CD18) to the third immunoglobulin-like domain of ICAM-1 (CD54) and its regulation by glycosylationCell, 1991
- Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extensionGene, 1989