Antibodies against the major brain isofbrms of 14‐3‐3 protein
Authors:
- Harry MartinHMHarry Martin
- Alastair AitkenAAAlastair Aitken
- 4 October 1993
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 331 (3) , 296-303
Abstract
14‐3‐3 proteins are apparently ubiquitous eukaryotic proteins that comprise a large number of isoforms. They have been implicated in the regulation of a wide range of biological processes [reviewed in Aitken et al. (1992) Trends Biochem. Sci. 17,498‐501]. We have raised specific antibodies against each mammalian brain isoform of 14‐3‐3 employing peptides synthesised from the amino‐tenninal regions. The peptides were, like the proteins from mammalian brain, N‐acetylated. The antiserum specific for the epsilon isoform did not recognise the recombinant form of this protein (lacking the N‐acetyl co‐translational modification) expressed inE. coliuntil it was chemically acetylated.Keywords
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