Identification of Purple Acid Phosphatases in Chickpea and Potential Roles of CaPAP7 in Seed Phytate Accumulation
Open Access
- 8 September 2017
- journal article
- research article
- Published by Springer Nature in Scientific Reports
- Vol. 7 (1) , 1-12
Abstract
Purple acid phosphatases (PAPs) play important roles in phosphate (Pi) acquisition and utilization. These PAPs hydrolyze organic Phosphorus (P) containing compounds in rhizosphere as well as inside the plant cell. However, roles of PAPs in one of the most widely cultivated legumes, chickpea (Cicer arietnum L.), have not been unraveled so far. In the present study, we identified 25 putative PAPs in chickpea (CaPAPs) which possess functional PAP motifs and domains. Differential regulation of CaPAPs under different nutrient deficiencies revealed their roles under multiple nutrient stresses including Pi deficiency. Interestingly, most of the CaPAPs were prominently expressed in flowers and young pods indicating their roles in flower and seed development. Association mapping of SNPs underlying CaPAPs with seed traits revealed significant association of low Pi inducible CaPAP7 with seed weight and phytate content. Biochemical characterization of recombinant CaPAP7 established it to be a functional acid phosphatase with highest activity on most abundant organic-P substrate, phytate. Exogenous application of recombinant CaPAP7 enhanced biomass and Pi content of Arabidopsis seedlings supplemented with phytate as sole P source. Taken together, our results uncover the PAPs in chickpea and potential roles of CaPAP7 in seed phytate accumulation.Keywords
This publication has 64 references indexed in Scilit:
- The Arabidopsis Purple Acid Phosphatase AtPAP10 Is Predominantly Associated with the Root Surface and Plays an Important Role in Plant Tolerance to Phosphate LimitationPlant Physiology, 2011
- Identification of soybean purple acid phosphatase genes and their expression responses to phosphorus availability and symbiosisAnnals of Botany, 2011
- De Novo Assembly of Chickpea Transcriptome Using Short Reads for Gene Discovery and Marker IdentificationDNA Research, 2011
- Biochemical and Molecular Characterization of PvPAP3, a Novel Purple Acid Phosphatase Isolated from Common Bean Enhancing Extracellular ATP UtilizationPlant Physiology, 2009
- Approaches and challenges to engineering seed phytate and total phosphorusPlant Science, 2009
- Molecular and Biochemical Characterization of AtPAP15, a Purple Acid Phosphatase with Phytase Activity, in ArabidopsisPlant Physiology, 2009
- Overexpressing AtPAP15 Enhances Phosphorus Efficiency in SoybeanPlant Physiology, 2009
- Activation of β-Glucan Synthases by Wall-Bound Purple Acid Phosphatase in Tobacco CellsPlant Physiology, 2009
- An Arabidopsis Purple Acid Phosphatase with Phytase Activity Increases Foliar AscorbatePlant Physiology, 2007
- Biochemical and Molecular Characterization of AtPAP26, a Vacuolar Purple Acid Phosphatase Up-Regulated in Phosphate-Deprived Arabidopsis Suspension Cells and SeedlingsPlant Physiology, 2006