EVALUATION OF AMOXICILLIN WITHDRAWAL PERIOD TIME IN POMPANO LIVER AND KIDNEY

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Abstract
Amoxicillin is an antibiotic which belongs to the group of penicillins. It is approved in Taiwan for treating bacterial infections caused by Streptococcus sp. and Photobacterium sp. in anguilliformes, perciformes and salmoniformes. The pharmacokinetics of amoxicillin were determined in pompano following oral administration of a single dose of 40 mg/kg. Residue studies were performed to determine residues in liver and kidney tissues of healthy fish after oral gavage of amoxicillin at a daily dose of 40 mg/kg for five consecutive days. Amoxicillin residues were analyzed by HPLC using Hypersil-100 C18 column (150 mm × 4.6 mm i.d.) and mobile phase consists of 10 mM K2HPO4(pH 8.5) with acetonitrile (80:20, v/v), at a flow rate of 1 mL/min. The effluent was monitored using a fluorescence detector set as 358 and 440 nm as excitation and emission wavelengths. Following a single oral dose, amoxicillin residues in 0.5 h post-dosing pompano were at a maximum of 6.17 μg/g in liver and 4.27 μg/g in kidney; the concentration of amoxicillin in liver and kidney declined with half-lives of 18.3 and 12.0 h. Amoxicillin residues in pompano liver and kidney tissues were proved to be under the MRL, 0.5 ug/g (liver and kidney) after a withdrawal period of five days.

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