Targeted isolation and cloning of 100-kb microbial genomic sequences by Cas9-assisted targeting of chromosome segments
Authors:
- 21 April 2016
- journal article
- Published by Springer Nature in Nature Protocols
- Vol. 11 (5) , 960-975
Abstract
Here the authors provide the procedural details of a new approach to clone large (up to 100 kb) microbial genomic sequences using Cas9-assisted targeting of chromosome segments (CATCH). The Cas9 cleavage is achieved in agarose gel, and the cleaved product can be ligated into a cloning vector.Keywords
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