CD34 − /CD133 + /VEGFR-2 + Endothelial Progenitor Cell Subpopulation With Potent Vasoregenerative Capacities
Authors:
- 17 February 2006
- journal article
- research article
- Published by Wolters Kluwer Health in Circulation Research
- Vol. 98 (3) , e20-5
Abstract
Our goal was to identify functionally important subpopulations within the heterogenous group of endothelial progenitor cells (EPC). Fluorescence-activated cell sorter analysis of CD133 + progenitor cells revealed the presence of CD34 + and CD34 − subpopulations. CD34 − /133 + progenitors differentiate into CD34 + /133 + EPC, adhere more potently than these in response to SDF-1, and rapidly home to sites of limb ischemia in human volunteers. In human coronary atherectomy samples, fewer CD34 − /133 + than CD34 + /133 + EPC are present in stable plaques, whereas cell numbers increase with a reversion of the ratio in unstable lesions. In CD34 − /133 + EPC-injected nude mice, more transplanted cells coexpressing endothelial markers home to carotid artery lesion endothelium than in CD34 + /133 + -injected mice. In the former, lesions were smaller and reendothelialization higher than in the latter. We identified a new CD34 − /133 + EPC subpopulation, which is apparently a precursor of “classical” CD34 + /133 + EPC, and functionally more potent than these with respect to homing and vascular repair.Keywords
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