Cubic Na0.5Bi0.5TiO3 nanoperovskite significantly expands the application of sensitive immunosensor for the detection of carcinoembryonic antigen

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Abstract
Nanosized sodium bismuth perovskite titanate (NBT) was synthesized and first used as the electrochemical immune sensing platform for the sensitive detection of carcinoembryonic antigen (CEA). Gold nanoparticles (Au NPs) grew on the surface of NBT through forming Au-N bond to obtain Au@NBT, and a label-free electrochemical immunosensor was proposed using Au@NBT as an immunosensing recognizer towards CEA. The well-ordered crystal structure of NBT was not changed at all after the modification of Au NPs outside, but significantly improved the conductivity, catalytic activity, and biocompatibility of the Au@NBT-modified electrode. The unique cubic crystal nanostructure of NBT offered a large active area for both Au NP modification and the subsequent immobilization of biomolecules over the electrode surface, triggering the effective generation of promising properties of the proposed Au@NBT-based electrochemical immunosensor. As expected, favorable detection performances were achieved using this immunosensor towards CEA detection, where a good linear relationship between the current response and CEA concentration was obtained in the concentration range 10 fg mL−1 to 100 ng mL−1 with a low detection limit (LOD) of 13.17 fg mL−1. Also, the significantly enhanced selectivity, and stability guaranteed the promising electrochemical properties of this immunosensor. Furthermore, the analysis of real serum samples verified the high feasibility of this new method in clinical CEA detection. This work opens a new window for the application of nanoperovskite in the early detection of CEA. Graphical abstract
Funding Information
  • Science and Technology Project of China Minmetals Corporation (2022ZXA02)
  • Medical-Engineering Interdisciplinary Project of University of Shanghai for Science and Technology (1022310503,1021341405)
  • Shanghai Collaborative Innovation Center of Energy Therapy for Tumors (Shanghai Collaborative Innovation Center of Energy Therapy for Tumors)
  • Scientific research program of Shanghai Science and Technology Commission (21140903200)
  • Fundamental Research Funds for Central Universities of the Central South University (2022-4-YB-17)

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