Interaction of Myelin Basic Protein with Myelin-like Lipid Monolayers at Air–Water Interface

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Abstract
The interaction of myelin basic protein (MBP) and the myelin sheath of the central nervous system is related to neurodegenerative diseases like multiple sclerosis. To get more insights into this interaction the adsorption of MBP to model lipid monolayers similar to the myelin of the central nervous system is studied at the air-water interface with monolayer adsorption experiments. Measuring the surface pressure and the related maximum insertion pressure of MBP for different myelin-like lipid monolayers provides information about the role of the single lipids in the myelin. Depending on the ratio of negatively charged lipids to neutrally charged lipids and the distance between charges the adsorption process is determined by two counteracting effects: i) protein incorporation resulting in an increasing surface pressure and ii) lipid condensation due to electrostatic interaction between the positively charged protein and negatively charged lipids resulting in a decreasing surface pressure. While electrostatic interactions lead to high insertion pressures, the associated lipid condensation has a lowering effect on the fuidity of the myelin-like monolayer.
Funding Information
  • Deutsche Forschungsgemeinschaft (HI1094/7-1)

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