Anti-HLA antibodies of predetermined specificity: a chemically synthesized peptide induces antibodies specific for HLA-A,B heavy chain.
Authors:
- W R ChurchWCW R Church
- L E WalkerLWL E Walker
- R A HoughtenRHR A Houghten
- R A ReisfeldRRR A Reisfeld
- 1 January 1983
- journal article
- research article
- Published by National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (1) , 255-258
Abstract
A chemically synthesized dodecapeptide (Asp-Ala-Ala-Ser-Pro-Arg-Glu-Glu-Pro-Arg-Ala-Pro), consisting of residues 39-50 of the HLA-B7 H chain sequence and containing a highly hydrophilic hexapeptide segment (Pro-Arg-Glu-Glu-Pro-Arg), induced antibodies that bound specifically to free HLA-A and HLA-B H chains and to the soluble dodecapeptide. Although these antibodies reacted with free HLA-A, B H chains in blots on nitrocellulose, they failed to immunoprecipitate the HLA-A, B-.beta.2-microglobulin antigen complex solubilized from human lymphoblastoid cells by nonionic detergents. Apparently, the physical characteristics of this particular soluble hydrophilic dodecapeptide closely resemble nonallotypic determinants expressed by free HLA-A, B H chains, and such antigenic sites may be modified by conformational changes in the native HLA-A, B H chain-.beta.2-microglobulin complex.Keywords
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