Highly Sensitive Determination of microRNA Using Target‐Primed and Branched Rolling‐Circle Amplification
Authors:
- 15 April 2009
- journal article
- research article
- Published by Wiley in Angewandte Chemie International Edition
- Vol. 48 (18) , 3268-3272
Abstract
One‐nucleotide differences in microRNAs (miRNAs) can be discriminated in an assay based on a branched rolling‐circle amplification (BRCA) reaction and fluorescence quantification. With the proposed method miRNA can be detected at concentrations as low as 10 fM, and the miRNA in a total RNA sample of a few nanograms can be determined. magnified imageKeywords
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