CEL-Seq2—Single-Cell RNA Sequencing by Multiplexed Linear Amplification
- 27 April 2019
- book chapter
- Published by Springer Nature
- Vol. 1979, 45-56
Abstract
Single-cell RNA sequencing has revolutionized the way we look at cell populations. Of the methods available, CEL-Seq was the first to use linear RNA amplification. With early barcoding and 3′ sequencing, it is sensitive, cost-effective and easy to perform. Here we describe a protocol for performing CEL-Seq2 on sorted cells, which can be performed without any special equipment.Keywords
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