Monoclonal Antibodies Against Herpes Simplex Virus Type 1-infected Nuclei Defining and Localizing the ICP8 Protein, 65K DNA-binding Protein and Polypeptides of the ICP35 Family

Authors:
  • PSP. Schenck
    P. Schenck
  • SPS. Pietschmann
    S. Pietschmann
  • HGH. Gelderblom
    H. Gelderblom
  • GPG. Pauli
    G. Pauli
  • HLH. Ludwig
    H. Ludwig
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Abstract
Summary The production and properties of monoclonal antibodies raised against herpes simplex virus type 1 (HSV-1)-infected cell nuclei are described. Biological and immunochemical assays revealed that these antibodies recognize four different proteins in HSV-1-infected cells. Four antibodies reacted with the major DNA-binding protein (ICP8) and six with the 65K DNA-binding protein. Two antibodies detected the ICP35 family of proteins and one antibody bound to a protein with an apparent mol. wt. of 60K. Immune electron microscopy showed that the major DNA-binding protein had a patchy distribution, whereas the 65K DNA-binding protein was evenly spread in the infected cell nuclei. The 60K protein as well as the polypeptides of the ICP35 family were preferentially found associated with the viral capsid.

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