Knockdown of Radixin by RNA interference Suppresses the Growth of Human Pancreatic Cancer Cells in Vitro and in Vivo

Authors:
  • MSMao-Min Song
    Mao-Min Song
  • ZZZhi-Qiang Zhong
    Zhi-Qiang Zhong
  • NLNa Li
    Na Li
  • PWPi-Lin Wang
    Pi-Lin Wang
  • SCShi Cheng
    Shi Cheng
  • RBRi-Xing Bai
    Ri-Xing Bai
  • HYHui-Sheng Yuan
    Hui-Sheng Yuan
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Abstract
Knockdown of Radixin by RNA interference Suppresses the Growth of Human Pancreatic Cancer Cells in Vitro and in Vivo Pancreatic neoplasms;small interference RNA;radixin; Radixin, encoded by a gene on chromosome 11, plays important roles in cell motility, invasion and tumor progression. However, its function in pancreatic cancer remains elusive. In this study, radixin gene expression was suppressed with a lentivirus-mediated short-hairpin RNA (shRNA) method. We found that radixin shRNA caused down-regulation of radixin in PANC-1 cells, associated with inhibition of pancreatic cancer cell proliferation, survival, adhesion and invasive potential in vitro. When radixin-silenced cells were implanted in nude mice, tumor growth and microvessel density were significantly inhibited as compared to blank control cells or nonsense shRNA control cells. Thrombospondin-1 (TSP-1) and E-cadherin were up-regulated in radixin-silenced PANC-1 cells. Our results suggest that radixin might play a critical role in pancreatic cancer progression, possibly through invvolvement of down-regulation of TSP-1 and E-cadherin expression.

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