МУТАНТНЫЙ ФАКТОР ИНИЦИАЦИИ EIF4A (R362Q) НЕ ПОДАВЛЯЕТ СБОРКУ 48S ПРЕИНИЦИАТОРНОГО КОМПЛЕКСА НА МРНК С ЛИДИРУЮЩЕЙ ПОСЛЕДОВАТЕЛЬНОСТЬЮ МРНК, КОДИРУЮЩЕЙ ОБЕЛИН, "Молекулярная биология"

Authors:
Publisher website
Google Scholar
Abstract
The formation of ribosomal 48S initiation complexes at the start codon of the mRNA leader sequence that encodes obelin has been studied using the method of primer extension inhibition (toeprinting). Experiments have been performed in a system composed of purified individual components required to initiate translation. The influence of the dominant negative mutant of factor eIF4A (R362Q) on translation initiation has been studied. It has been shown that the presence of the mutant in reaction mixture has no effect on efficiency of formation of the 48S complexes at start codon of the template studied.