Induction and Rejoining of DNA Double-Strand Breaks in Bladder Tumor Cells

Authors:
  • VMV. J. McKelvey-Martin
    V. J. McKelvey-Martin
  • DHD. G. Hirst
    D. G. Hirst
  • SMS. R. McKeown
    S. R. McKeown
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Abstract
Price, M. E., McKelvey-Martin, V. J., Robson, T., Hirst, D. G. and McKeown, S. R. Induction and Rejoining of DNA Double-Strand Breaks in Bladder Tumor Cells. The induction and rejoining of radiation-induced double-strand breaks (DSBs) in cells of six bladder tumor cell lines (T24, UM-UC-3, TCC-SUP, RT112, J82, HT1376) were measured using the neutral comet assay. Radiation dose–response curves (0–60 Gy) showed damage (measured as mean tail moment) for five of the cell lines in the same rank order as cell survival (measured over 0–10 Gy), with the least damage in the most radioresistant cell line. Damage induction correlated well with clonogenic survival at high doses (SF10) for all six cell lines. At the clinically relevant dose of 2 Gy, correlation was good for four cell lines but poor for two (TCC-SUP and T24). The rejoining process had a fast and slow component for all cell lines. The rate of these two components of DNA repair did not correlate with cell survival. However, the time taken to red...

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