In VitroSynthesis of Dehydroepiandrosterone Sulfate by an Adrenal Adenoma1

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Abstract
Tritium-labeled 17α-hydroxypregnenolone and dehydroepiandrosterone were incubated, respectively, with slices and a microsome-free supernatant of a human adrenal adenoma which was removed from a patient with hirsutism and masculinization. The percentage of conversion from 17αhydroxypregnenolone to dehydroepiandrosterone sulfate was 10.6 (with ACTH) and 13.2 (without ACTH) with the slices. There was a 35.1 % conversion of dehydroepiandrosterone to dehydroepiandrosterone sulfate with the microsome-free supernatant system. Radiochemical homogeneity of the isolated dehydroepiandrosterone sulfate was verified by paper chromatography, paper electrophoresis, countercurrent distribution, column chromatography and, after solvolysis, by characterization of the free steroid. The study indicates that a steroid sulfokinase (or sulfokinases) is present in human adrenal adenomatous tissue.

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