Isolation and Manipulation of Mouse Trophoblast Stem Cells
Authors:
Open Access
- 15 October 2008
- journal article
- unit
- Published by Wiley in Current Protocols in Stem Cell Biology
- Vol. 7 (1) , 1E.4.1-1E.4.27
Abstract
The isolation of stable trophoblast stem (TS) cell lines from early mouse embryos has provided a useful cell culture model to study trophoblast development. TS cells are derived from pre‐implantation blastocysts or from the extraembryonic ectoderm of early post‐implantation embryos. The derivation and maintenance of mouse TS cells is dependent upon continuous fibroblast growth factor (FGF) signaling. Gene expression analysis, differentiation in culture, and chimera formation show that TS cells accurately model the mouse trophoblast lineage. This unit describes how to derive, maintain, and manipulate TS cells, including DNA transfection and chimera formation. Curr. Protoc. Stem Cell Biol. 7:1E.4.1‐1E.4.27. © 2008 by John Wiley & Sons, Inc.Keywords
This publication has 35 references indexed in Scilit:
- Lymphoid Enhancer Factor 1-Mediated Wnt Signaling Promotes the Initiation of Trophoblast Lineage Differentiation in Mouse Embryonic Stem CellsStem Cells, 2008
- Loss of the maternal imprint in Dnmt3L mice leads to a differentiation defect in the extraembryonic tissueDevelopmental Biology, 2006
- EuthanasiaCurrent Protocols in Immunology, 2006
- Interaction between Oct3/4 and Cdx2 Determines Trophectoderm DifferentiationCell, 2005
- Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocystDevelopment, 2005
- Imprinted X-inactivation in extra-embryonic endoderm cell lines from mouse blastocystsDevelopment, 2005
- Maintenance of mouse trophoblast stem cell proliferation by TGF-β/activinDevelopmental Biology, 2004
- Spatial and temporal patterns of ERK signaling during mouse embryogenesisDevelopment, 2003
- Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptidesNature, 1988
- Establishment in culture of pluripotential cells from mouse embryosNature, 1981