Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization
Authors:
- 1 September 1987
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 53 (9) , 2256-2259
Abstract
A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DNA from beta-hemolytic (CAMP-positive) strains of L. monocytogenes hybridized with this probe.Keywords
This publication has 13 references indexed in Scilit:
- Tn916-induced mutations in the hemolysin determinant affecting virulence of Listeria monocytogenesJournal of Bacteriology, 1987
- Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenesInfection and Immunity, 1986
- Isolation of Listeria monocytogenes from raw milkApplied and Environmental Microbiology, 1986
- Production of listeriolysin by beta-hemolytic strains of Listeria monocytogenesInfection and Immunity, 1986
- Pasteurized Milk as a Vehicle of Infection in an Outbreak of ListeriosisNew England Journal of Medicine, 1985
- An improved colony hybridization method with significantly increased sensitivity for detection of single genesPlasmid, 1983
- Experimental infection of mice with Listeria monocytogenes and L. innocua.1980
- Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.Proceedings of the National Academy of Sciences, 1975
- Purification and Characterization of Hemolysin Produced by Listeria monocytogenesAmerican Journal of Veterinary Research, 1974
- PURIFICATION OF THE SOLUBLE HEMOLYSINS OF LISTERIA MONOCYTOGENESJournal of Bacteriology, 1964