CLONING AND CHROMOSOMAL LOCALIZATION OF HUMAN GENES ENCODING THE 3 CHAINS OF TYPE-VI COLLAGEN

Authors:
  • MMMG MATTEI
    MG MATTEI
  • EPE PASSAGE
    E PASSAGE
  • NVN VANCONG
    N VANCONG
  • DPD PRIBULACONWAY
    D PRIBULACONWAY
  • KMK MANN
    K MANN
  • RDR DEUTZMANN
    R DEUTZMANN
  • RTR TIMPL
    R TIMPL
  • MCML CHU
    ML CHU
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  • 1 March 1988
    • journal article
    • research article
Abstract
Type VI collagen is a heterotrimer composed of three polypeptide chains, .alpha.1(VI), .alpha.2(VI), and .alpha.3(VI). By immunological screening of an expression cDNA library, human cDNAs specific for each chain were isolated and characterized. Major mRMNA species encoding these chains have a size of 4.2 kb (.alpha.1), 3.5 kb (.alpha.2), and 8.5 kb (.alpha.3). The cDNA clones were also used to map the genes on human chromosomes by somatic cell hybrid analysis and in situ hybridization. The .alpha.1(VI) and .alpha.2(VI) collagen genes were both located on chromosome 21, in band q223. This represents a third example of a possible physical proximity of two collagen loci. The .alpha.3(VI) collagen gene were localized to chromosome 2, in the region 2q37. The .alpha.3(VI) collagen gene is the fifth extracellular matrix gene to be localized to 2q, as four other extracellular matrix genes.sbd.i.e., that .alpha.1(III) and .alpha.2(V) collagene genes, the elastin gene, and the fibronectin gene.sbd.have been previously mapped to the distal region of the long arm of chromosome 2.

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