Extraction Procedures for Use Prior to HPLC Nucleotide Analysis Using Microparticle Chemically Bonded Packings

Authors:
  • SCShow-Chuen Chen
    Show-Chuen Chen
  • PBPhyllis R. Brown
    Phyllis R. Brown
  • DRDouglas M. Rosie
    Douglas M. Rosie
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Abstract
In preparing nucleotide extracts for HPLC analysis using microparticle, chemically bonded packings, tri-octyl-aminefreon solutions can be used for the extraction of the acid from the solution if the concentration of the amine is carefully controlled.