Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death
- 4 June 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (17) , 3515-3524
Abstract
Microtubules are polymers of α/β‐tubulin participating in essential cell functions. A multistep process involving distinct molecular chaperones and cofactors produces new tubulin heterodimers competent to polymerise. In vitro cofactor A (TBCA) interacts with β‐tubulin in a quasi‐native state behaving as a molecular chaperone. We have used siRNA to silence TBCA expression in HeLa and MCF‐7 mammalian cell lines. TBCA is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. In MCF‐7 cells, cell death was preceded by a change in cell shape resembling differentiation.Keywords
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