Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI)
Open Access
- 19 August 2010
- journal article
- Published by Optica Publishing Group in Optics Express
- Vol. 18 (18) , 18875-18885
Abstract
Uperresolution Optical Fluctuation Imaging (SOFI) as initially demonstrated allows for a resolution enhancement in imaging by a factor of square-root of two. Here, we demonstrate how to increase the resolution of SOFI images by re-weighting the Optical Transfer Function (OTF). Furthermore, we demonstrate how cross-cumulants can be exploited to obtain a fair approximation of the underlying Point-Spread Function. We show a two-fold increase of resolution (over the diffraction limit) of near-infrared quantum dot labeled tubulin-network of 3T3 fibroblastsKeywords
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