Single Cell Genomics: Advances and Future Perspectives
Open Access
- 30 January 2014
- journal article
- review article
- Published by Public Library of Science (PLoS) in PLOS Genetics
- Vol. 10 (1) , e1004126
Abstract
Advances in whole-genome and whole-transcriptome amplification have permitted the sequencing of the minute amounts of DNA and RNA present in a single cell, offering a window into the extent and nature of genomic and transcriptomic heterogeneity which occurs in both normal development and disease. Single-cell approaches stand poised to revolutionise our capacity to understand the scale of genomic, epigenomic, and transcriptomic diversity that occurs during the lifetime of an individual organism. Here, we review the major technological and biological breakthroughs achieved, describe the remaining challenges to overcome, and provide a glimpse into the promise of recent and future developments.Keywords
This publication has 120 references indexed in Scilit:
- Somatic copy number mosaicism in human skin revealed by induced pluripotent stem cellsNature, 2012
- Single-Neuron Sequencing Analysis of L1 Retrotransposition and Somatic Mutation in the Human BrainCell, 2012
- Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cellsNature Biotechnology, 2012
- Genome-wide Single-Cell Analysis of Recombination Activity and De Novo Mutation Rates in Human SpermCell, 2012
- Detectable clonal mosaicism from birth to old age and its relationship to cancerNature Genetics, 2012
- Detectable clonal mosaicism and its relationship to aging and cancerNature Genetics, 2012
- Single-Cell Exome Sequencing Reveals Single-Nucleotide Mutation Characteristics of a Kidney TumorCell, 2012
- Single-cell dissection of transcriptional heterogeneity in human colon tumorsNature Biotechnology, 2011
- Whole-genome molecular haplotyping of single cellsNature Biotechnology, 2011
- The cancer genomeNature, 2009