Systematic Determination of Transcription Factor DNA-Binding Specificities in Yeast
- 1 January 2016
- book chapter
- Published by Springer Nature
- Vol. 1361, 203-225
Abstract
Understanding how genes are regulated, decoding their “regulome”, is one of the main challenges of the post-genomic era. Here, we describe the in vitro method we used to associate cis-regulatory sites with cognate trans-regulators by characterizing the DNA-binding specificity of the vast majority of yeast transcription factors using Protein Binding Microarrays. This approach can be implemented to any given organism.Keywords
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